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rpmi 1640 media  (ATCC)


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    ATCC rpmi 1640 media
    Rpmi 1640 Media, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/media+rpmi+1640/Jurkat%2C+Clone+E6-1/us12655229-655-15-11
    Average 99 stars, based on 4458 article reviews
    rpmi 1640 media - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: The PIP4K2 inhibitor THZ-P1-2 exhibits antileukemia activity by disruption of mitochondrial homeostasis and autophagy
    Article Snippet: .. Cells were cultured in appropriate media (RPMI-1640, IMDM, or alpha-MEM) supplemented with 10 or 20% fetal bovine serum (FBS) according to the ATCC and DSMZ recommendations, plus 1% penicillin/streptomycin and maintained at 5% CO 2 and 37°C. .. THZ-P1-2 was obtained from MedChemExpress (Monmouth Junction, NJ, USA) and diluted to a 25 mM stock solution in dimethyl sulfoxide (Me 2 SO 4 ; DMSO).

    Article Title: Pharmacological Inhibition of PIP4K2 Potentiates Venetoclax-Induced Apoptosis in Acute Myeloid Leukemia
    Article Snippet: .. Cells were cultured in appropriate media (RPMI-1640, IMDM, or alpha-MEM), supplemented with 10 or 20% of fetal bovine serum (FBS) according to the ATCC’s and DSMZ’s recommendations, plus 1% of penicillin/streptomycin, and maintained at 5% of CO 2 and 37°C. .. THZ-P1-2 was obtained from MedChemExpress (Monmouth Junction, NJ, USA) and diluted to 25 mM in dimethyl sulfoxide (Me 2 SO 4 ; DMSO).

    Article Title: Comprehensive analysis of cytoskeleton regulatory genes identifies ezrin as a prognostic marker and molecular target in acute myeloid leukemia.
    Article Snippet: Purpose Despite great advances that have been made in the understanding of the molecular complexity of acute myeloid leukemia (AML), very little has been translated into new therapies.. Here, we set out to investigate the impact of cytoskeleton regulatory genes on clinical outcomes and their potential as therapeutic targets in AML.. Methods Gene expression and clinical data were retrieved from The Cancer Genome Atlas (TCGA) AML study and used for survival and functional genomics analyses.

    Article Title: Cellular and Molecular Effects of Eribulin in Preclinical Models of Hematologic Neoplasms
    Article Snippet: SUP-B15 was kindly provided by Dr. Lucas Eduardo Botelho de Souza (National Institute of Science and Technology in Stem Cells and Cell Therapy, Ribeirão Preto, São Paulo, Brazil). .. The cells were cultured in an appropriate media (RPMI-1640, IMDM, or alpha-MEM) supplemented with 10 or 20% FBS according to the American Type Culture Collection (ATCC) or Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ) recommendations, plus 1% penicillin/streptomycin. ..

    Article Title: Metabolic reprogramming represents a targetable mechanism to overcome acquired resistance to venetoclax in acute myeloid leukemia.
    Article Snippet: Acute myeloid leukemia (AML) often develops resistance to the BCL2 inhibitor venetoclax through metabolic reprogramming.. This study established acquired venetoclax-resistant AML models (MV4-11VR and MOLM13VR) to explore resistance mechanisms and therapeutic strategies.. Cell viability and apoptosis assays revealed robust acquired resistance to venetoclax upon intermittent drug exposure.

    Article Title: The PIP4K2 inhibitor THZ-P1-2 exhibits antileukemia activity by disruption of mitochondrial homeostasis and autophagy.
    Article Snippet: .. Cells were cultured in appropriate media (RPMI-1640, IMDM, or alpha-MEM) supplemented with 10 or 20% fetal bovine serum (FBS) according to the ATCC and DSMZ recommendations, plus 1% penicillin/streptomycin, and maintained at 5% CO2 and 37 °C. .. THZ-P1-2 was obtained from MedChemExpress (Monmouth Junction, NJ, USA) and diluted to 25mM in dimethyl sulfoxide (Me2SO4; DMSO).

    Suspension:

    Article Title: Enabling In Vivo Photocatalytic Activation of Rapid Bioorthogonal Chemistry by Repurposing Silicon-Rhodamine Fluorophores as Cytocompatible Far-Red Photocatalysts.
    Article Snippet: .. The cell suspension was centrifuged at 450 rpm for 5 min and a total of 450k cells were resuspended into media (193 μL, RPMI-1640, ATCC) and then mixed with a PBS solution of DHTz (6), SiR (1d), catalase and H2O2 at a desired final concentration for each component: A. Catalase: 2 mM 6, 40 μM 1d, 2.5 mg/mL catalase B. .. The cell suspension was centrifuged at 450 rpm for 5 min and a total of 450k cells were resuspended into media (193 μL, RPMI-1640, ATCC) and then mixed with a PBS solution of DHTz (6), SiR (1d), catalase and H2O2 at a desired final concentration for each component: A. Catalase: 2 mM 6, 40 μM 1d, 2.5 mg/mL catalase B.

    Concentration Assay:

    Article Title: Enabling In Vivo Photocatalytic Activation of Rapid Bioorthogonal Chemistry by Repurposing Silicon-Rhodamine Fluorophores as Cytocompatible Far-Red Photocatalysts.
    Article Snippet: .. The cell suspension was centrifuged at 450 rpm for 5 min and a total of 450k cells were resuspended into media (193 μL, RPMI-1640, ATCC) and then mixed with a PBS solution of DHTz (6), SiR (1d), catalase and H2O2 at a desired final concentration for each component: A. Catalase: 2 mM 6, 40 μM 1d, 2.5 mg/mL catalase B. .. The cell suspension was centrifuged at 450 rpm for 5 min and a total of 450k cells were resuspended into media (193 μL, RPMI-1640, ATCC) and then mixed with a PBS solution of DHTz (6), SiR (1d), catalase and H2O2 at a desired final concentration for each component: A. Catalase: 2 mM 6, 40 μM 1d, 2.5 mg/mL catalase B.



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    Image Search Results


    A . Western blot for HSP60 and IFNα in whole cell lysates of naïve mouse neutrophils (neut.) or alveolar macrophages (AM). B-D . Immunofluorescence confocal microscopy of naïve mouse neutrophils (B), human neutrophils (C), or Hoxb8 neutrophils (D) at 20X (top panel) or 60X (bottom panel) magnification. Images show DNA (Hoechst), IFNα, and fibrillarin as indicated. E . FACS analysis of intracellular IFNα expression in GR-1 + MPO lo and GR-1 + MPO hi Hoxb8 neutrophils. F . Immunofluorescence confocal microscopy of HoxB8 neutrophils ranked by immature to mature nuclear morphology. Images show DNA (Hoechst) and IFNα. G . Western blot for HSP60 and IFNα in streptavidin (SA) pull down and flow through of GFP-APEX2-NIK3x-expressing HoxB8 neutrophils and progenitor cells following proximity biotinylation. H . Immunofluorescence confocal microscopy of mouse alveolar macrophages. Images show DNA (Hoechst), IFNα, and fibrillarin. I . ImageJ quantitation for percent fibrillarin area in total nuclear area of naïve mouse alveolar macrophage and neutrophils. J-K . qRT-PCR of 45S pre-rRNA in naïve mouse alveolar macrophage and neutrophils (J), or in HoxB8 progenitors (Day 0) and Hoxb8 neutrophils (Day 4) (K). Each data point is one field of view (I) or an independent well of infected cells (J-K) from at least two independent experiments. Error bars represent the mean ± SEM. Statistical differences between neutrophil and alveolar macrophage, or between HoxB8 progenitors and HoxB8 neutrophils, were performed using Student’s t-test: *p < 0.05, **p-value < 0.01.

    Journal: bioRxiv

    Article Title: Neutrophils rapidly release nucleoli-stored IFNα during infection

    doi: 10.64898/2026.05.18.726057

    Figure Lengend Snippet: A . Western blot for HSP60 and IFNα in whole cell lysates of naïve mouse neutrophils (neut.) or alveolar macrophages (AM). B-D . Immunofluorescence confocal microscopy of naïve mouse neutrophils (B), human neutrophils (C), or Hoxb8 neutrophils (D) at 20X (top panel) or 60X (bottom panel) magnification. Images show DNA (Hoechst), IFNα, and fibrillarin as indicated. E . FACS analysis of intracellular IFNα expression in GR-1 + MPO lo and GR-1 + MPO hi Hoxb8 neutrophils. F . Immunofluorescence confocal microscopy of HoxB8 neutrophils ranked by immature to mature nuclear morphology. Images show DNA (Hoechst) and IFNα. G . Western blot for HSP60 and IFNα in streptavidin (SA) pull down and flow through of GFP-APEX2-NIK3x-expressing HoxB8 neutrophils and progenitor cells following proximity biotinylation. H . Immunofluorescence confocal microscopy of mouse alveolar macrophages. Images show DNA (Hoechst), IFNα, and fibrillarin. I . ImageJ quantitation for percent fibrillarin area in total nuclear area of naïve mouse alveolar macrophage and neutrophils. J-K . qRT-PCR of 45S pre-rRNA in naïve mouse alveolar macrophage and neutrophils (J), or in HoxB8 progenitors (Day 0) and Hoxb8 neutrophils (Day 4) (K). Each data point is one field of view (I) or an independent well of infected cells (J-K) from at least two independent experiments. Error bars represent the mean ± SEM. Statistical differences between neutrophil and alveolar macrophage, or between HoxB8 progenitors and HoxB8 neutrophils, were performed using Student’s t-test: *p < 0.05, **p-value < 0.01.

    Article Snippet: Transduced HoxB8 progenitor cells were maintained in 5% CO 2 at 37°C, in HoxB8 progenitor media (RPMI 1640 media containing 10% FBS, 10ng/ml G-CSF (MedChem Express), 1□μM β-estradiol (Sigma), and 10 ng/ml stem cell factor (SCF) from conditioned supernatant of SCF-producing Chinese hamster ovary (CHO) cells kindly provided by Dr. David Sykes (Massachusetts General Hospital, MA)).

    Techniques: Western Blot, Immunofluorescence, Confocal Microscopy, Expressing, Quantitation Assay, Quantitative RT-PCR, Infection